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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
06/08/2015 |
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Data da última atualização: |
10/03/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BARBOSA, N. C. S.; SCHNADELBACH, A. S.; LEITE, K. R. B.; JESUS, O. N. de; ABREU, E. F. M.; BARBOSA, C. de J. |
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Afiliação: |
NAIRA COSTA SOARES BARBOSA, UFBA; ALESSANDRA SELBACH SCHNADELBACH, UFBA; KELLY REGINA BATISTA LEITE, UFBA; ONILDO NUNES DE JESUS, CNPMF; EMANUEL FELIPE MEDEIROS ABREU, CNPMF; CRISTIANE DE JESUS BARBOSA, CNPMF. |
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Título: |
Microscopia de varredura da superfície foliar de espécies de passifloraceae infectadas com o Cowpea aphid-borne mosaic virus (CABMV). |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 3., 2014, Santos. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2014. |
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Idioma: |
Português |
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Conteúdo: |
Avaliou-se cinco espécies de Passiflora, sendo uma comercial, Passiflora edulis f. flavicarpa Deg., e quatro silvestres, P. cincinnata Mast., P. gibertii N.E.Br., P. maliformis Vell. e P. setacea DC., do Banco Ativo de Germoplasma da Embrapa Mandioca e Fruticultura Tropical. Destas, a espécie comercial é suscetível ao CABMV, enquanto as silvestres são consideradas mais resistentes. De cada espécie foram analisadas folhas de três indivíduos de plantas sadias e três inoculados mecanicamente em condições de casa de vegetação, além de três naturalmente infectadas em campo. As superfícies adaxiais e abaxiais das folhas foram observadas sob microscópio eletrônico de varredura do Serviço de Microscopia Eletrônica da Fiocruz-BA. Observou-se a presença de cristaloides de cera sobre as cutículas em ambas as faces das quatro espécies silvestres, estando ausentes na espécie comercial. Tal característica pode estar relacionada à resistência ao CABMV nas espécies silvestres. |
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Palavras-Chave: |
Passionfruits. |
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Thesagro: |
Maracujá; Melhoramento vegetal; Vírus. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/127558/1/MICROSCOPIA-DE-VARREDURA.pdf
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Marc: |
LEADER 01790nam a2200217 a 4500
001 2021525
005 2016-03-10
008 2014 bl uuuu u00u1 u #d
100 1 $aBARBOSA, N. C. S.
245 $aMicroscopia de varredura da superfície foliar de espécies de passifloraceae infectadas com o Cowpea aphid-borne mosaic virus (CABMV).$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 3., 2014, Santos. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos$c2014
520 $aAvaliou-se cinco espécies de Passiflora, sendo uma comercial, Passiflora edulis f. flavicarpa Deg., e quatro silvestres, P. cincinnata Mast., P. gibertii N.E.Br., P. maliformis Vell. e P. setacea DC., do Banco Ativo de Germoplasma da Embrapa Mandioca e Fruticultura Tropical. Destas, a espécie comercial é suscetível ao CABMV, enquanto as silvestres são consideradas mais resistentes. De cada espécie foram analisadas folhas de três indivíduos de plantas sadias e três inoculados mecanicamente em condições de casa de vegetação, além de três naturalmente infectadas em campo. As superfícies adaxiais e abaxiais das folhas foram observadas sob microscópio eletrônico de varredura do Serviço de Microscopia Eletrônica da Fiocruz-BA. Observou-se a presença de cristaloides de cera sobre as cutículas em ambas as faces das quatro espécies silvestres, estando ausentes na espécie comercial. Tal característica pode estar relacionada à resistência ao CABMV nas espécies silvestres.
650 $aMaracujá
650 $aMelhoramento vegetal
650 $aVírus
653 $aPassionfruits
700 1 $aSCHNADELBACH, A. S.
700 1 $aLEITE, K. R. B.
700 1 $aJESUS, O. N. de
700 1 $aABREU, E. F. M.
700 1 $aBARBOSA, C. de J.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
11/02/2016 |
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Data da última atualização: |
12/02/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MENDES, L. W.; MENDES, R.; TSAI, S. M. |
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Afiliação: |
L. W. MENDES, CENA/USP; Microbial Ecology Department, Netherlands Institute of Ecology, Wageningen, The Netherlands; RODRIGO MENDES, CNPMA; S. M. TSAI, CENA/USP. |
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Título: |
Analysis of the rhizosphere microbial communities of common beans resistant and susceptible to Fusarium oxysporum. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 0568-1. |
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Idioma: |
Inglês |
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Conteúdo: |
The rhizosphere is the narrow zone of soil around the living plant roots that is influenced by the activity of the plants. Many beneficial microorganisms in the rhizosphere provide plants with mineral nutrients, phytohormones, and also help to protect the plant against soil-borne pathogens. Microbiological studies are addressed to understand how rhizosphere microorganisms are recruited from soil and either benefit or harm plant growth, nutrition and health. Here, we aimed to identify potential microbial groups and functional traits correlated to the suppression of the soil borne pathogen Fusarium oxysporum, the causal agent of Fusarium wilt on common beans. We used shotgun metagenomics to investigate the rhizosphere microbiome of two common bean cultivars classified as resistant (Milênio) and susceptible (Alvorada). Plants were grown in mesocosms experiments in Amazon Dark Earth, a soil with high microbial diversity. Chemical analysis indicated a significant increase of pH, Ca, Fe, Zn, Mn, B, nitrate, cation exchange capacity, sum of bases and base saturation in rhizosphere of both common bean types. The microbial community structure of rhizosphere was different from the bulk soil in a deeper taxonomic classification (genera), revealing the selection process in this environment. Quantitative PCR showed an increase of 16S rRNA copy number with the increase resistance to the fungus in ADE soil. The resistant cultivar presented higher taxonomic diversity but lower functional diversity. The most abundant phyla in rhizosphere were Proteobacteria (41%), Actinobacteria (31%), Firmicutes (5%), Acidobacteria (3%) and Chloroflexi (3%). The resistant cultivar presented more abundance of the phyla Chlamydiae, Spirochaetes, Deinococcus-Thermus and Chrysiogenetes in comparison to the susceptible one and bulk soil. Comparing the resistant to the susceptible cultivar in a finer taxonomic level, 24 genera presented higher abundance in the resistant one, highlighting Bacillus and Pseudomonas. Preliminary analysis showed that there is a specific selection of the microbial communities inhabiting the rhizosphere of a resistant common bean cultivar. Further analysis will combine 16S rRNA gene sequencing and metatranscriptome for a deep taxonomical and functional analysis. MenosThe rhizosphere is the narrow zone of soil around the living plant roots that is influenced by the activity of the plants. Many beneficial microorganisms in the rhizosphere provide plants with mineral nutrients, phytohormones, and also help to protect the plant against soil-borne pathogens. Microbiological studies are addressed to understand how rhizosphere microorganisms are recruited from soil and either benefit or harm plant growth, nutrition and health. Here, we aimed to identify potential microbial groups and functional traits correlated to the suppression of the soil borne pathogen Fusarium oxysporum, the causal agent of Fusarium wilt on common beans. We used shotgun metagenomics to investigate the rhizosphere microbiome of two common bean cultivars classified as resistant (Milênio) and susceptible (Alvorada). Plants were grown in mesocosms experiments in Amazon Dark Earth, a soil with high microbial diversity. Chemical analysis indicated a significant increase of pH, Ca, Fe, Zn, Mn, B, nitrate, cation exchange capacity, sum of bases and base saturation in rhizosphere of both common bean types. The microbial community structure of rhizosphere was different from the bulk soil in a deeper taxonomic classification (genera), revealing the selection process in this environment. Quantitative PCR showed an increase of 16S rRNA copy number with the increase resistance to the fungus in ADE soil. The resistant cultivar presented higher taxonomic diversity but lower functional di... Mostrar Tudo |
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Palavras-Chave: |
Shotgun metagenomics; Soil born pathogen. |
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Thesagro: |
Feijão; Fusarium Oxysporum. |
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Thesaurus NAL: |
microbial ecology; microbiome. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/138852/1/2015RA-073.pdf
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Marc: |
LEADER 03030nam a2200205 a 4500
001 2036665
005 2016-02-12
008 2015 bl uuuu u00u1 u #d
100 1 $aMENDES, L. W.
245 $aAnalysis of the rhizosphere microbial communities of common beans resistant and susceptible to Fusarium oxysporum.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 0568-1.$c0568
520 $aThe rhizosphere is the narrow zone of soil around the living plant roots that is influenced by the activity of the plants. Many beneficial microorganisms in the rhizosphere provide plants with mineral nutrients, phytohormones, and also help to protect the plant against soil-borne pathogens. Microbiological studies are addressed to understand how rhizosphere microorganisms are recruited from soil and either benefit or harm plant growth, nutrition and health. Here, we aimed to identify potential microbial groups and functional traits correlated to the suppression of the soil borne pathogen Fusarium oxysporum, the causal agent of Fusarium wilt on common beans. We used shotgun metagenomics to investigate the rhizosphere microbiome of two common bean cultivars classified as resistant (Milênio) and susceptible (Alvorada). Plants were grown in mesocosms experiments in Amazon Dark Earth, a soil with high microbial diversity. Chemical analysis indicated a significant increase of pH, Ca, Fe, Zn, Mn, B, nitrate, cation exchange capacity, sum of bases and base saturation in rhizosphere of both common bean types. The microbial community structure of rhizosphere was different from the bulk soil in a deeper taxonomic classification (genera), revealing the selection process in this environment. Quantitative PCR showed an increase of 16S rRNA copy number with the increase resistance to the fungus in ADE soil. The resistant cultivar presented higher taxonomic diversity but lower functional diversity. The most abundant phyla in rhizosphere were Proteobacteria (41%), Actinobacteria (31%), Firmicutes (5%), Acidobacteria (3%) and Chloroflexi (3%). The resistant cultivar presented more abundance of the phyla Chlamydiae, Spirochaetes, Deinococcus-Thermus and Chrysiogenetes in comparison to the susceptible one and bulk soil. Comparing the resistant to the susceptible cultivar in a finer taxonomic level, 24 genera presented higher abundance in the resistant one, highlighting Bacillus and Pseudomonas. Preliminary analysis showed that there is a specific selection of the microbial communities inhabiting the rhizosphere of a resistant common bean cultivar. Further analysis will combine 16S rRNA gene sequencing and metatranscriptome for a deep taxonomical and functional analysis.
650 $amicrobial ecology
650 $amicrobiome
650 $aFeijão
650 $aFusarium Oxysporum
653 $aShotgun metagenomics
653 $aSoil born pathogen
700 1 $aMENDES, R.
700 1 $aTSAI, S. M.
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Embrapa Meio Ambiente (CNPMA) |
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